Metaproteomics Approach to Gut Microbiota in Health and Disease
Bernardo Petriz, Filipe Moura and Octávio Franco
from: Microbiota: Current Research and Emerging Trends (Edited by: Takashi Matsumoto and Yoshio Yamaoka,). Caister Academic Press, U.K. (2019) Pages: 55-68.
Abstract
Phylotype profiling is conducted in a variety of biomedical research areas with significant impact on human health. So far, metagenomics has provided meaningful data linking the microbiota profile to the host health. However, profiling of the gut microbiome is limited to the identification of its constituents and their diversity. However, the complex molecular interactions between the microbiome and the gut environment affect the host metabolism, immunity, and homeostasis. These interactions are being investigated by metaproteomics and metabolomics, as analytical approaches in the identification of large-scale proteins and metabolites, providing informative details and functional aspects of the microbiota and the gut environment. As a complementary approach to metagenomic and metatranscriptomic data, the metaproteomic analysis may also provide some new insights into the influence of the gut microbiota over the host biology. Considering the significant clinical potential of this field, the metaproteomic studies in the gut microbiota are still unexplored. In this chapter, we will present the main aspects and limitations of metaproteomics, as well as the perspectives in the analysis of the gut microbiome proteins in health and disease read more ...
