Now available on the web: A comparison of the following
PCR machines lists various features to help you decide which qPCR instrument is most suitable for your needs.
- Applied Biosystems: ABI 7300, ABI 7500, ABI 7500 Fast, ABI 7900 Fast HT with automation accessory, ABI StepOne
- Roche: LightCycler 480, LightCycler 1.5, LightCycler 2.0
- Stratagene: Mx4000, Mx3000P, Mx3005P
- Cepheid: SmartCycler
- Corbett: Rotor-Gene 6000
- Eppendorf: Mastercycler ep realplex
- BioRad: MiniOpticon, MyiQ, Opticon2, Chromo4, iQ5
read more ...Bibliography:
- Real-Time PCR: Current Technology and Applications
- Real-Time PCR in Microbiology: From Diagnosis to Characterization
- PCR Troubleshooting: The Essential Guide
- PCR Books
Labels: instruments, molecular machines, platforms, qPCR, real-time PCR
ABC transporters are fascinating molecular machines that use the energy of ATP to catalyze the transport of a tremendous variety of substrates across biological membranes in a vectorial fashion. All ABC transporters analyzed so far are composed of two nucleotide-binding domains (NBD) and two transmembrane domains (TMD) that can be arranged in any possible combination. However, additional transmembrane segments or extended NBDs, raise the possibility that these extensions act as platforms to interact with additional proteins with functional or regulatory consequences.
Recent crystal structures of isolated NBDs in different functional states and the structures of intact
ABC transporters have elucidated the three-dimensional architecture, domain-domain interactions and putative signaling pathways in these membrane proteins that guarantee efficient substrate recognition and translocation in an ATP-dependent manner.
from Thorsten Jumpertz, I. Barry Holland and Lutz Schmitt (2009)
in ABC Transporters in Microorganisms Edited by Alicia Ponte-Sucre, Caister Academic Press. ISBN: 978-1-904455-49-3
Further reading:
ABC Transporters as Targets against Drug ResistanceLabels: molecular machines, nucleotide-binding domains, translocation, two transmembrane domains