Caister Academic Press

Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications

Authors: Chih-Hsuan TsaiMolecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan, Republic of China, and Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China, and Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China Sung-Chan WeiInstitute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China, and Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China Huei-Ru LoInstitute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China Yu-Chan ChaoMolecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan, Republic of China, and Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, Republic of China, and Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan, Republic of China, and Department of Plant Pathology and Microbiology, College of Bioresources and Agriculture, National Taiwan University, Taipei, Taiwan, Republic of China, and Department of Life Sciences, College of Life Sciences, National Chung Hsing University, Taichung, Taiwan, Republic of China
Abstract:
Open-access article
The baculovirus-insect cell system has long been deployed for a variety of applications including for use as biopesticides, for recombinant protein production, transient transgene expression, tissue therapy, and for vaccine production. Apart from the advantage of large-scale heterologous protein production with appropriate eukaryotic post-translational modification, foreign proteins can also be displayed on the viral envelope. This surface-display technology preserves the native multimeric structure of the protein, thereby expanding the clinical and pharmaceutical utility of the baculovirus system. Recombinant baculoviruses displaying major antigens for human or animal viruses can serve as appropriate vaccines. They can also serve as effective diagnostic platforms and various cell-based assay systems. In this review, we discuss progress in applying baculovirus surface-display, including protein display on the envelope, capsid, and occlusion bodies of baculoviruses, as well as on cells. We will also describe strategies for improvement of this biotechnological approach.
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