Caister Academic Press

An Overview of Real-Time PCR Platforms

Authors: J. M. J. Logan and K. J. Edwards
Abstract: Real-time PCR continues to have a major impact across many disciplines of the biological sciences and this has been a driver to develop and improve existing instruments. From the first two commercial platforms introduced in the mid 1990s, there is now a choice in excess of a dozen instruments, which continues to increase. Advances include faster thermocycling times, higher throughput, flexibility, expanded optical systems, increased multiplexing and more user-friendly software. In this chapter the main features of each instrument are compared and factors important to weigh up when deciding on a platform are highlighted.
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