PCR Troubleshooting and Optimization Figure
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Edited by: Suzanne Kennedy and Nick Oswald Published: 2011 ISBN: 978-1-904455-72-1 An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ... |
Figure from: PCR Troubleshooting and Optimization
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 9. Figure 6. Melt-curves of amplified DNA targets. Semi-quantitative estimates of the amount of target DNA (non-methylated vs. methylated) present after amplification can be determined by comparing the derivative peak heights, relative to local background signal amplitude, of the non-methylated amplicon which melts at 80.8 C and its methylated cognate which melts at 84.9 C. Shown are representative control melt curves of mixtures independently amplified non-methylated and methylated versions of the same locus after bisulfite conversion.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.
