PCR Troubleshooting and Optimization Figure
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Edited by: Suzanne Kennedy and Nick Oswald Published: 2011 ISBN: 978-1-904455-72-1 An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ... |
Figure from: PCR Troubleshooting and Optimization
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 9. Figure 15. The NCode miRNA Universal qRT-PCR workflows. miRNAs are polyadenylated in a carefully optimized, combined reverse transcription step using the SuperScript VILO enzyme formulation and a universal primer. The universal first-strand cDNAs correspond to miRNAs, other non-coding RNAs and mRNAs which can all be analyzed subsequently.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.
