PCR Troubleshooting and Optimization Figure
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Edited by: Suzanne Kennedy and Nick Oswald Published: 2011 ISBN: 978-1-904455-72-1 An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ... |
Figure from: PCR Troubleshooting and Optimization
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 6. Figure 2. Emission spectra, and spectral overlap, of four commonly used fluorescent dyes (FAM, HEX, ROX, and Cy5). To deconvolute the signal of multiple dyes within a single channel, dye calibration information is used to remove all fluorescent-signal present in a given channel that does not belong to the dye of interest. In short, dye calibration information is used to subtract, using simultaneuous equations, any dye bleed-through emissions (denoted by an asterisk above) from the emission signal generated by the dye of interest.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.
