PCR Troubleshooting and Optimization Figure
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Edited by: Suzanne Kennedy and Nick Oswald Published: 2011 ISBN: 978-1-904455-72-1 An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ... |
Figure from: PCR Troubleshooting and Optimization
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 4. Figure 7. Melting curves and normalized derivative melting peaks showing the difference that primer purification can make. HPLC-purified primers were used in the clean peak sample whereas desalted only primers were used in the sample with a high temperature shoulder, which results from additional undesired product generation.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.
