PCR Troubleshooting and Optimization Figure
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Edited by: Suzanne Kennedy and Nick Oswald Published: 2011 ISBN: 978-1-904455-72-1 An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ... |
Figure from: PCR Troubleshooting and Optimization
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 4. Figure 1. Schematic representing key considerations of PCR assay design and optimization. Although the optimization process is illustrated as stepwise, it is also iterative. For example, once an optimal magnesium concentration is identified, it is wise to re-identify an optimal annealing temperature and re-examine the limit of detection.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.
