PCR Troubleshooting and Optimization Figure
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Edited by: Suzanne Kennedy and Nick Oswald Published: 2011 ISBN: 978-1-904455-72-1 An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ... |
Figure from: PCR Troubleshooting and Optimization
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 1. Figure 2. Cross-sectional diagram of a rapid, air-controlled thermal cycler. An Idaho Technology ATC 1605 thermal cycler from 1990 is shown. PCR was performed in glass capillaries which were placed in a cylindrical air chamber. A central halogen lamp functioned as a heater and an internal fan ensured even temperature distribution within the chamber. For cooling, room temperature air was drawn through the chamber by opening a door (top right). The use of air as both a heating and cooling medium allowed for rapid heat transfer to the capillaries, resulting in 30-cycle amplification times of 10-30 min.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.
