PCR Troubleshooting and Optimization Figure
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Edited by: Suzanne Kennedy and Nick Oswald Published: 2011 ISBN: 978-1-904455-72-1 An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ... |
Figure from: PCR Troubleshooting and Optimization
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 11. Figure 8. Process flow from bulk solid-phase emPCR to next-gen sequencing. A. Bulk emulsified reaction in tube. B. Enlargement showing single reaction droplet, contaning DNA capture bead, single DNA template and PCR reagents prior to amplification. C. Depiction of the same droplet following amplification, wherein the DNA capute bead is now covered with immbolized copies of the amplified template. D. Emuslions are broken; bead population is comprised of both PCR(+) and PCR(-) beads as determined by limiting dilution of template DNA. E. Enrichment processes select for PCR (+) beads. F. PCR positive beads can then be sequenced by various Next-Gen sequencing systems (ABI SOLiD and 454 FLX shown).
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.
