PCR Troubleshooting and Optimization Figure
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Edited by: Suzanne Kennedy and Nick Oswald Published: 2011 ISBN: 978-1-904455-72-1 An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ... |
Figure from: PCR Troubleshooting and Optimization
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 10. Figure 3. Advanced software analysis for HRMA heterozygote scanning. When many samples are analyzed and multiple domains or alleles are present, curve overlay or "temperature shifting" increases accuracy. Curve shapes are compared by shifting the temperature axis to superimpose the curves, usually over a low fluorescence segment. Samples should also be analyzed without curve overlay for homozygote detection. Shown is a 544 bp PCR product that melts in two domains, with an SNP revealed in the lower temperature domain.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.
