PCR Troubleshooting and Optimization Figure
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Edited by: Suzanne Kennedy and Nick Oswald Published: 2011 ISBN: 978-1-904455-72-1 An essential book for all scientists using PCR, real-time PCR, qPCR and related techniques. read more ... |
Figure from: PCR Troubleshooting and Optimization
Full details of this book at PCR Troubleshooting and Optimization. More figures at PCR Figures.
Chapter 10. Figure 1. Dual hybridization probe (HybProbe) technology for SNP genotyping. Two probes, each with one fluorophore are designed to hybridize next to each other on the target so that they are in resonance energy transfer. One probe is blocked at the 3'-end and hybridizes to invariant sequence (anchor probe) while the other hybridizes over the variable locus (sensor probe). After PCR, the solution is heated and fluorescence monitored. A melting curve for a heterozygote is shown, along with the corresponding negative derivative plot.
Further reading at PCR Troubleshooting and Optimization. More figures at PCR Figures.
