Acanthamoeba: Biology and Pathogenesis
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Author: Naveed Khan Published: 2009 ISBN: 978-1-904455-43-1 Everything that is known about Acanthamoeba is critically reviewed and divided into easy-to-follow sections. The current state of research on genomics, molecular and cellular biology, life cycles, geographical distribution, role in ecosystem, morphology, motility, phylogenetics, genotyping, metabolism, regulation of morphogenesis, host-parasite interactions, the molecular and immunological basis of pathogenesis, methods of transmission, epidemiology, clinical manifestation, diagnosis, treatment, new target development and drug resistance, and much more. read more ... |
Figure from: Acanthamoeba Biology and Pathogenesis
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Section C. Figure 7. Flow cytometry analysis of specificities of Acanthamoeba castellanii (T4 genotype)-specific phage clone with (A) A. castellanii (T4 genotype); (B) A. astronyxis (T7 genotype). Acanthamoeba (106) were incubated with phage clone (1012), washed and resuspended in sheep anti-M13 antibody. Following this, cells were washed, resuspended in fluorescein isothiocyanate conjugated anti-sheep IgG (10 microgram/ml) and analyzed by flow cytometry. Note that mean channel fluorescence is higher in pathogenic A. castellanii (T4 genotype) than weak-pathogenic A. astronyxis.
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