Caister Academic Press

Preface to Quantitative Real-time PCR in Applied Microbiology

Molecular Diagnostics: Current Research and Applications
Edited by: Jim Huggett and Justin O'Grady
"I would highly recommend this book" (Doodys); "useful new insights" (Aus. J. Med. Sci.)
Since its introduction in the mid-1990s, quantitative real-time PCR (qPCR) has revolutionized almost all areas of microbiology. It has become a common technique used in most laboratories interested in clinical, environmental, food and industrial microbiology. This versatile technology keeps evolving, and new platforms, chemistries, approaches and software are becoming available, making qPCR more sensitive, faster and affordable than ever before.

This book aims to provide all microbiologists, both novice and experienced users, with the latest information available on qPCR technology. The initial chapters cover the main technical aspects of qPCR, from a thorough description of the technique to choosing an instrument, fluorescent chemistries, experimental approaches, controls, validation and software. In the second part of the book, the focus is put on how to design qPCR assays for quantifying microorganisms from different substrates and conditions in a cultureindependent manner. Finally, the final chapters focus on microbial gene expression and describe the use of RT-qPCR for quantifying transcripts from complex environmental conditions and for validating microbial microarray data. Future trends in RT-qPCR are also discussed, as well as their implication in applied microbiology. I hope that this book will find its place on the bench in microbiology laboratories, so that users will come back to it from time to time, perhaps stimulating the development of novel innovative qPCR approaches to address their specific needs read more ...

Martin Filion