Proteome Analysis of Yersinia pestis and the Mammalian Host Response to Y. pestis Infection
Rembert Pieper and Scott N. Peterson
from: Yersinia: Systems Biology and Control (Edited by: Elisabeth Carniel and B. Joseph Hinnebusch). Caister Academic Press, U.K. (2012)
In the last few years, proteomic analyses of Y. pestis have been focused on the identification of proteins derived from in vitro cultures that sought to mimic important aspects of in vivo environments and have provided valuable insight into dynamic abundance profiles of numerous proteins and their subcellular localizations, comparing growth conditions relevant in the context of the flea vector/mammalian host life cycle. A proteomic analysis of Y. pestis under iron starvation conditions revealed marked changes in the utilization of pathways related to energy metabolism, stress response and Fe-S cluster assembly. Virulence-associated factors were often found to be highly expressed only under specific environmental conditions. This included T3SS subunits (such as LcrV and YopD), Pla protease, the phospholipase D2 Ymt, the outer membrane (OM) receptor for iron/yersiniabactin, the β-barrel OM adhesion protein Ail and the capsular F1 antigen. Some of these proteins were also major antigens (Caf1, LcrV, YopD and YopE) although the human immune response appears to target a larger variety of Y. pestis proteins than the aforementioned virulence factors read more ...