Characterization of Microbial Community Structures in Recreational Waters and Primary Sources of Fecal Pollution with a Next-Generation Sequencing Approach
Orin C. Shanks, Sandra McLellan, Susan M. Huse and Mitchell L. Sogin
from: Environmental Microbiology: Current Technology and Water Applications (Edited by: Keya Sen and Nicholas J. Ashbolt). Caister Academic Press, U.K. (2011)
The invention of new approaches to DNA sequencing commonly referred to as next generation sequencing technologies is revolutionizing the study of microbial diversity. In this chapter, we discuss the characterization of microbial population structures in surface waters and potentially contributing fecal sources using a GS-FLX pyrosequencer. An overview of the principals of the technology, workflow, sequencing strategies, and data analysis challenges are included. In addition, the bacterial population structure of an untreated wastewater sewage sample is reported and discussed to demonstrate a pyrosequencing approach for studying a microbial community. Emphasis is placed on the application of pyrosequencing technology for recreational water quality management including the discovery of alternative indicators of fecal pollution and improving the link between fecal pollution and the transmission of waterborne disease read more ...