Identity and Function of Single Microbial Cells Within a Community by Raman Microspectroscopy and Related Single-cell Techniques
Daniel S. Read and Andrew S. Whiteley
from: Environmental Microbiology: Current Technology and Water Applications (Edited by: Keya Sen and Nicholas J. Ashbolt). Caister Academic Press, U.K. (2011)
Linking both identity and function within microbial communities has long been seen as essential for understanding the role that bacteria play in the environment. Techniques based on the study of single microbial cells offer a unique approach that provides information about heterogeneity within populations, and the role of spatial organization within the environment. This chapter details some of the prominent single-cell techniques currently in use for the study of microbial ecology, with a particular focus on Raman spectroscopy. A general overview of this technique is provided, with examples of its applicability for studying different features of microbial systems. Special attention is given to the use of Raman spectroscopy in combination with Fluorescence in situ Hybridization (FISH) and Stable Isotope Probing (SIP), which together can be utilized to gain an insight into the identity and function of single bacterial cells in situ read more ...