Quantitative Proteomics in Vaccine Research
Massimiliano Biagini and Nathalie Norais
from: Advanced Vaccine Research Methods for the Decade of Vaccines (Edited by: Fabio Bagnoli and Rino Rappuoli). Caister Academic Press, U.K. (2015) Pages: 75-102.
Functional genomics and proteomics have become power tools for all life science researchers and the application in the vaccinology field is particular intriguing. The urgent need for fast, effective and safer vaccines let a substantial shift towards quantitative approaches that will have a huge impact in all vaccine development stages. Proteomics is deeply contributing in the early phase of candidate discovery and it appears more and more implemented in the following phases of antigen characterization, formulation, safety and conservation in strains circulating in epidemic areas. In this chapter we will virtually retrace the brief history of proteomics, following the technical steps that have characterized the evolution of this "daughter" of functional genomics, deepening the studies that have contributed to the knowledge of pathogen biology and the microbiological and immunological mechanisms, fundamental for the development of new vaccines. And more importantly you will appreciate as precise quantitative analysis can now be carried out thanks to the latest advances in mass spectrometry. We will see how proteomic technologies have become an essential tool for the analysis of vaccines in the manufacturing phase. Finally, we will evocate the last great challenge in the field of vaccinology, the unification of diverse scientific knowledge to have a unitary vision of the relationship between human being immune system and the pathogen by System Biology read more ...