Deep Sequencing in Vaccine Research, Development and Post-marketing Surveillance
Stefano Censini, Silvia Guidotti, Giulia Torricelli, Rino Rappuoli and Fabio Bagnoli
from: Advanced Vaccine Research Methods for the Decade of Vaccines (Edited by: Fabio Bagnoli and Rino Rappuoli). Caister Academic Press, U.K. (2015) Pages: 3-32.
Since it was established in the ‘70s by Frederick Sanger DNA sequencing has brought enormous contribution to virtually all areas of life and medical sciences. More recently, Next Generation Sequencing technologies (NGS) have gradually flanked and then replaced the Sanger method in most sequencing projects. The major aim of this chapter is to describe the extraordinary technological advances from the first to the third generation sequencing as well as the consequent expanding range of their applications. NGS is enabling affordable large-scale genomics, metagenomics, transcriptomics and epigenomics studies at extremely deep resolution. Another key area of NGS is the analysis of human B- and T-lymphocyte repertoires. The explosion of new sequencing technologies was paralleled by a similar advance in computational science and synthetic biology leading to the era of "teleporting life". Altogether these new technologies and their applications are significantly increasing chances to discover and develop efficacious vaccines read more ...