Virus-induced gene silencing (VIGS) and its applications
Deep Ratan Kumar, Tejbhan Saini and Radhamani Anandalakshmi
from: Genes, Genetics and Transgenics for Virus Resistance in Plants (Edited by: Basavaprabhu L. Patil). Caister Academic Press, U.K. (2018) Pages: 277-328.
During the last two decades, virus-induced gene silencing (VIGS) has emerged as the most powerful and cost effective tool to determine gene functions in a relatively shorter period of time. VIGS has been used for both forward and reverse genetics studies for functional analysis of a large number of genes in both monocot and dicot plants. Various VIGS vectors have been developed, tested and modified to down-regulate the endogenous gene expression effectively in a sequence-specific manner in model plants, cereals, vegetables, fiber crops, fruits and timber trees. This review focuses on VIGS as a technique for inducing gene silencing in plants and its applications in understanding the role of genes controlling cellular and metabolic pathways, development, abiotic and biotic stress in economically important crop species read more ...