Management of geminiviruses focussing on small RNAs in tomato
Archana Singh and Sunil Kumar Mukherjee
from: Genes, Genetics and Transgenics for Virus Resistance in Plants (Edited by: Basavaprabhu L. Patil). Caister Academic Press, U.K. (2018) Pages: 235-254.
Geminiviruses are plant-infecting, ssDNA containing viruses, which have twinned geminate particles. Majority of geminiviruses belongs to the genus Begomovirus having either monopartite or bipartite genome (DNA-A and DNA-B). Tomato leaf curl New Delhi virus (ToLCNDV), a pathogenic member of genus Begomovirus, causes Tomato leaf curl disease (ToLCD). ToLCD is responsible for economic losses of up to 100% in many regions of world. Control of ToLCV is very difficult as the classical vector control method turns out to be an unworthy option and Ty-based breeding yields partial success. Our laboratory has employed RNAi-based pathogen derived resistance (PDR) approach to successfully tackle ToLCD. We have overproduced artificial microRNA (a-miR) and artificial tasiRNA (a-tasiRNA) in tomato to silence the RNAi-suppressors encoded by ToLCVs. A few of the tomato transgenics show high level of virus resistance when challenged with broad range of ToLCVs read more ...