Genetic engineering for virus resistance in plants: principles and methods
Basavaprabhu L. Patil
from: Genes, Genetics and Transgenics for Virus Resistance in Plants (Edited by: Basavaprabhu L. Patil). Caister Academic Press, U.K. (2018) Pages: 103-118.
Viral diseases are a major menace for cultivation of crop plants across the globe. Non-availability of virucides and sources of host-plant resistance for their introgression in farmers preferred cultivars has put up major challenge for control of plant viral diseases. Hence genetic engineering for development of virus resistant transgenic crop plants is a promising option when compared to other conventional tools and techniques. Since the first report of virus resistant transgenic in 1986, significant progress has been made in understanding the molecular basis of virus resistance and also in the tools and techniques used for plant genetic engineering. Despite major advancement in the area of plant biotechnology, in the last 30 years, there has been no significant increase in the deployment of virus resistant transgenic crops, except for few examples such as papaya. Thus to help the plant biologists, here in this chapter we have compiled and briefly discuss all the tools and techniques available for engineering virus resistance in crop plants. Of all the strategies currently available for engineering virus resistance, RNA-interference-based technology has shown greater success. The recently developed genome editing technology has also given new hope for virus resistant transgenic crops. Without compromising the safety aspects of these transgenic crops the regulatory procedures followed in the approval of virus resistant transgenic crops for farmer's cultivation have to be refined to ensure that the benefit reaches to the famers without much delay read more ...