Genetic Manipulation of Staphylococcus aureus
Peter J. McNamara
from: Staphylococcus: Molecular Genetics (Edited by: Jodi Lindsay). Caister Academic Press, U.K. (2008)
Genetic manipulation of S. aureus has provided an avenue for the discovery of new approaches to treat S. aureus infections, methods that are based on a molecular understanding of virulence and pathogenesis. This chapter provides basic information on strains of S. aureus and the tools and techniques that have been successfully employed to engineer their genome. The topics that are covered include: choosing a strain for study; the cultivation and husbandry of S. aureus; vectors and their uses; methods to introduce DNA into S. aureus, and methods for the mutagenesis of S. aureus. With the information in this chapter, it is possible to design experiments to examine a broad range of biological phenomena that will contribute to understanding the biology of S. aureus and the factors that make this bacteria such a formidable pathogen read more ...