Whole Genomes: Sequence, Microarray and Systems Biology
Matthew T. G. Holden and Jodi A. Lindsay
from: Staphylococcus: Molecular Genetics (Edited by: Jodi Lindsay). Caister Academic Press, U.K. (2008)
Whole genome technologies facilitate the investigation of the genetic processes that underpin bacterial cells. Whole genome sequences for twelve diverse Staphylococcus aureus isolates are available and their annotation provides enormous insight into S. aureus physiology, capabilities and virulence. Whole genome microarrays, built using the sequences, have enabled whole genome regulatory responses to environmental conditions or global regulators to be investigated. Comparative genomics by sequencing and by multi-strain microarrays have identified the S. aureus population structure and how genomes vary, as well as suggesting that invasive isolates do not carry more virulence genes than carriage isolates. Whole genomes provide the framework for other systems biology approaches such as RNomics, proteomics and metabolomics. In a rapidly changing field, this chapter summarises the major achievements so far, and what is likely to be achieved in the near future read more ...