Expression of Recombinant Proteins Using Bacillus subtilis Spores
Luis Carlos de Souza Ferreia and Wolfgang Schumann
from: Bacterial Spores: Current Research and Applications (Edited by: Ernesto Abel-Santos). Caister Academic Press, U.K. (2012)
Surface display of peptides and proteins on recombinant phages and bacterial cells has developed into a powerful technique to identify proteins with desired properties with multiple applications. This technique is of utmost importance as a tool for fundamental and applied research in the fields of microbiology, biotechnology and vaccination. Another promising bioparticle are spores produced by Bacillus subtilis cells that allow fusion of recombinant proteins to one of several outer spore coat proteins. There are several reports were antigens and proteins of biotechnological interest have been successfully displayed on the outside of spores. A major advantage of spores over bacterial cells is their intracellular biogenesis that does not require specific secretion sequences of the cellular apparatus. Therefore, heterologous proteins do not have to cross membranes and correct folding of these proteins can be achieved with the aid of cytoplasmic folder chaperones read more ...