Properties and Detection Methods of Bacilli Spores in Food and in Medical Settings
Olga Tarasenko, Pierre Alusta, Sergey Kazakov and Kalle Levon
from: Bacterial Spores: Current Research and Applications (Edited by: Ernesto Abel-Santos). Caister Academic Press, U.K. (2012)
Numerous members of Bacillus and Clostridium genera are widely distributed in nature. Their control is considerably important since these species are responsible for a variety of food spoilage, foodborne illnesses, and food-poisoning problems, in addition to their potential use as biological warfare agents. The present study concentrates on the taxonomic Bacillus cereus group covering studies of genetics and morphology, including that of harmful bacteria. Moreover, several classical issues in spore resistance biology are being considered. Examining a number of reports will provide us with morphological properties of spores as well as their biochemical composition. We will then extrapolate on some of the findings, thus enabling us to determine the role of how to identify proteins in spore immunology and outline a possible issue in this regard. This paper presents an overview of different physicochemical techniques meaningful for spore detection and identification as a basis for biosensor construction and/or other detection techniques. Special attention is given to different methods of spore detection and identification including but not limited to microbiological, immunological, genetic, and radiometric methods, automation of assay procedures, biosensors as well as new approaches. Future directions for development and problems related to biosensors are discussed in the final part of this review read more ...