RNAi Gene Therapy to Control HIV-1 Infection
Ben Berkhout and Olivier ter Brake
from: RNA Interference and Viruses: Current Innovations and Future Trends (Edited by: Miguel Angel Martínez). Caister Academic Press, U.K. (2010)
RNA interference (RNAi) was discovered as cellular gene regulation mechanism in 1998, but several RNAi-based applications for gene silencing have already made it into clinical trials. We will discuss RNAi approaches to target pathogenic human viruses causing acute or chronic infections, with a focus on persistent HIV-1 infection that would most likely require an RNAi-based gene therapy. Viruses like HIV-1 are particularly difficult targets for RNAi-attack because they are escape-prone, which requires combinatorial RNAi strategies to prevent viral escape. The future of antiviral RNAi therapeutics is very promising, but it remains of critical importance to include many controls in pre-clinical test models to unequivocally demonstrate sequence-specific action of the RNAi inducers read more ...