Real-Time PCR: Application to Food Authenticity and Legislation
Gordon Wiseman
from: Real-Time PCR: Current Technology and Applications (Edited by: Julie Logan, Kirstin Edwards and Nick Saunders). Caister Academic Press, U.K. (2009)
Abstract
Real-time PCR is now an accepted analytical tool within the food industry. Its principal role has been one of assisting the legislative authorities, major manufacturers and retailers to confirm the authenticity of foods. The most obvious role is the detection and quantification of GMOs, but real-time PCR makes a significant contribution to many other areas of the food industry, including food safety and other speciality analyses such as the detection of common wheat adulteration in pasta and the detection of allergenic species. The role of quantitative real-time PCR in determining the actual amount of these materials, which are subject to considerable regulation, is discussed together with a consideration of the uncertainty of the methods read more ...
