Analysis of mRNA Expression by Real-Time PCR
Stephen A. Bustin and Tania Nolan
from: Real-Time PCR: Current Technology and Applications (Edited by: Julie Logan, Kirstin Edwards and Nick Saunders). Caister Academic Press, U.K. (2009)
Abstract
Its conceptual and practical simplicity, capacity for high throughput, and combination of high sensitivity with exacting specificity has made the fluorescence-based real-time reverse transcription polymerase chain reaction (qRT-PCR or RT-qPCR) today's method of choice for the quantification of RNA. The technology continues to evolve rapidly with the introduction of new protocols, enzymes, chemistries and instrumentation and has become the "Gold Standard" for a huge range of applications in basic research, molecular medicine, and biotechnology. Progress is increasingly associated with an increased appreciation of the limitations associated with this technology and the need for careful experimental design, application and validation read more ...
