Quantification of Microorganisms Using a Functional Gene Approach
Lia C.R.S. Teixeira and Etienne Yergeau
from: Quantitative Real-time PCR in Applied Microbiology (Edited by: Martin Filion). Caister Academic Press, U.K. (2012)
Quantitative polymerase chain reaction (qPCR) represents an effective method to quantify genes or transcripts within environmental samples. For that reason, qPCR has been widely used to characterize the functional patterns of complex microbial communities. In this chapter we summarize some recent applications of different qPCR approaches targeting functional genes encoding key enzymes in the N-, C- and S-cycles and also functional genes related to antibiotic resistance. We also point out some limitation of qPCR approaches. The ongoing development of new molecular techniques, like metagenomics, will have positive impacts on the specificity and the coverage of qPCR assays, since the availability of more sequence data will help to improve the design of primers targeting functional genes read more ...