Early Events in the Papillomaviral Lifecycle
Patricia M. Day and John T. Schiller
from: Papillomavirus Research: From Natural History To Vaccines and Beyond (Edited by: M. Saveria Campo). Caister Academic Press, U.K. (2006)
The chapter follows the chronological transit of the PV virion from the initial interaction with the cell surface until delivery into the nucleus. The interaction of the particle with the cell surface occurs largely via interaction of the major capsid protein, L1, with cell surface heparan sulfate proteoglycans. It is unclear if a secondary receptor is involved. It is also unclear if the minor capsid protein, L2, plays a role in cell surface interactions. Most PV types that have been examined appear to enter the cell via a clathrin-dependent endocytic pathway. Infection can be abrogated by inhibitors of this pathway and colocalization of capsid proteins with markers of the early and late endosomal compartments has been observed. It has recently been proposed that L2 can mediate the escape of the virus from the endosome by lysis of the limiting membrane. L2 and the genome traffic into the nucleus and colocalize at ND10. Localization to this domain is critical for efficient transcription of the PV genome read more ...