Genetic Tools for Pseudomonas
Kyoung-Hee Choi, Lily A. Trunck, Ayush Kumar, Takehiko Mima, RoxAnn R. Karkhoff-Schweizer and Herbert P. Schweizer
from: Pseudomonas: Genomics and Molecular Biology (Edited by: Pierre Cornelis). Caister Academic Press, U.K. (2008)
Abstract
Genetic tools are required to take full advantage of the wealth of information generated by genome sequencing efforts, and ensuing global gene and protein expression analyses. Although the development of genetic tools has generally not kept up with the sequencing pace, substantial progress has been made in this arena. PCR- and recombination-based strategies allowed construction of whole genome expression and transposon insertion libraries. Similar strategies combined with improved transformation protocols facilitate high-throughput construction of deletion alleles and development of a broad-host-range mini-Tn7 chromosome integration system. While to date most of these tools and methods have been developed for and applied in P. aeruginosa, they will most likely also be applicable to other Pseudomonas with appropriate modifications read more ...
