Dissecting the Actin Cytoskeleton of Entamoeba histolytica from a Genomic Perspective
Chung Chau Hon, Kumiko Nakada-Tsukui, Tomoyoshi Nozaki and Nancy Guillén
from: Anaerobic Parasitic Protozoa: Genomics and Molecular Biology (Edited by: C. Graham Clark, Patricia J. Johnson and Rodney D. Adam). Caister Academic Press, U.K. (2010)
Host tissue invasion by E. histolytica is driven by motility and phagocytosis, which are both regulated primarily by reorganization of the actin cytoskeleton. In this chapter, the structural and signaling components of the actin cytoskeleton from available genomes of three Entamoeba species, E. histolytica, E.dispar and E. invadens, are annotated manually and compared systematically. These protein families include the actin superfamily, actin nucleators, calponin-related actin-binding proteins (ABPs), gelsolin-related ABPs, myosins, small GTPases and their regulators, as well as phospholipid signaling regulators. Recent findings on these proteins in E. histolytica are also reviewed. With an emphasis on the reliability of manual annotations, we aim to give an overview of the existence and diversity of the actin cytoskeleton and its related proteins in E. histolytica. We also hope this chapter will provide useful information for researchers wishing to investigate the many uncharacterized actin-cytoskeletal components and their regulators in E. histolytica, which should shed light on the molecular mechanisms of the parasite's motility and its phagocytosis-driven tissue invasion read more ...