Protein Microarrays: A Versatile Tool for Scientific Discovery
Johnathan Neiswinger, Jiang Qian and Heng Zhu
from: Proteomics: Targeted Technology, Innovations and Applications (Edited by: Manuel Fuentes and Joshua LaBaer). Caister Academic Press, U.K. (2014)
Over the last quarter century, protein microarray technology has emerged as a prominent field in scientific study. The versatility of the platform, coupled with the ability to characterize thousands of proteins in a parallel and high-throughput manner, has resulted in great strides in our knowledge database. Microarrays consist of three classes, analytical, functional, and reverse-phase, all of which have their own place in biomedical research. Much progress in this technology has been made over the last decade as new methods, particularly in microarray fabrication, have been developed. This chapter will give an overview of new and founding technologies, complete with a series of examples from both the basic and clinical research fields, including: microarray fabrication, detection methods (label-dependent and independent), binding interactions, post-translational modifications (PTMs), host-microbe interactions, and cancer research read more ...