Genome-wide Approaches to Understand Multi-drug Resistance in Pathogenic Fungi
Amelie Waldin, Nitnipa Soontorngun, Sarah MacPherson, Sadri Znaidi, Martine Raymond and Bernard Turcotte
from: Pathogenic Fungi: Insights in Molecular Biology (Edited by: Gioconda San-Blas and Richard A. Calderone). Caister Academic Press, U.K. (2008)
A number of fungal species, such as Candida albicans, are human pathogens. Treatment with antifungal drugs quite often results in the appearance of resistant strains. Various mechanisms leading to resistance have been described. For example, a number of resistant clinical isolates overexpress genes encoding drug efflux pumps. Recent advances in molecular biology have allowed the study of the phenomenon of multi-drug resistance on a genome-wide scale. This chapter is aimed at first describing the use of DNA microarrays to study the expression profiling of pathogenic fungi. The second part of this review focuses on proteomic approaches used to determine variation of protein levels associated with the development of resistance to various antifungal drugs read more ...