The Molecular Biology of Porphyromonas gingivalis
Margaret J. Duncan
from: Molecular Oral Microbiology (Edited by: Anthony H. Rogers). Caister Academic Press, U.K. (2008)
Porphyromonas gingivalis is a Gram-negative oral anaerobe strongly associated with chronic adult periodontitis. The bacterium is relatively easy to grow, produces a number of well-characterized virulence factors, and can be manipulated genetically. The availability of the genome sequence will enhance our understanding of the biology of the P. gingivalis, and how it interacts with the environment, other bacteria, and the human host. Beginning with a brief description of the genome, this chapter proceeds with a discussion on strain variability, and the relationship of P. gingivalis to other oral and enteric Bacteroidetes. The genetic methods we use with P. gingivalis are based on those for the enteric Bacteroides, and the development and use of these systems is discussed. Among the most studied P. gingivalis genes are those that code for functions that either interact directly with the human host cells or protect the bacterium from host activities. Several recent studies are presented to illustrate the technologies and the scope of this research. Finally, new studies to determine the molecular mechanisms of gene regulation are described read more ...