Applied Genomics of Some Oral Bacteria
Stuart G. Dashper, Paul D. Veith, Ching Seng Ang, Peter S. Zilm and Eric C. Reynolds
from: Molecular Oral Microbiology (Edited by: Anthony H. Rogers). Caister Academic Press, U.K. (2008)
The simultaneous and inter-related rapid advances in genome sequencing, mass spectrometry and computing power have provided the tools for examining oral bacteria and their interactions with each other and the host at a global molecular level. In this chapter we focus on the development and usage of applied genomics techniques, mainly proteomics, for the study of Gram-negative anaerobic bacterial species found primarily in subgingival plaque. Proteomic analyses have already been applied to the discovery and characterization of proteins that comprise the proteome of Porphyromonas gingivalis and are now being applied to aid our understanding of the effects of environmental cues on the proteomes of oral bacteria, including Fusobacterium nucleatum, Tannerella forsythia and Treponema denticola. These species share the same environment and have been shown to interact with each other on a number of levels. We examine the range of currently-available proteomic methodologies that can be used to investigate the elaborate interactions between cells in the complex microbial biofilm communities found in the oral cavity and the interaction of these bacteria with the host read more ...