Genome-wide Analysis of Sense-antisense Transcripts
Chihiro Kohama and Hidenori Kiyosawa
from: Non-coding RNAs and Epigenetic Regulation of Gene Expression: Drivers of Natural Selection (Edited by: Kevin V. Morris). Caister Academic Press, U.K. (2012)
Genomic DNA and full-length cDNA sequencing projects have provided fundamental data that have led us to the novel notion that antisense transcription is a universal phenomenon in many organisms, including mammals and plants. Successive expression analyses utilizing microarrays, genome-tiling arrays, and recent RNA sequencing with next-generation sequencers have supported this concept. We review how these genome-wide, natural antisense transcript analyses have proceeded, and we then introduce representative examples of the known functions and functional implications of antisense transcripts. The variety of modes in which antisense transcripts function in cells reveals that these transcripts do not constitute a uniform group, but perform various types of gene expression regulation read more ...