Insights on Plant Development Using NGS Technologies
Ying Wang and Yuling Jiao
from: Next-generation Sequencing: Current Technologies and Applications (Edited by: Jianping Xu). Caister Academic Press, U.K. (2014)
Despite being a recent breakthrough technology, Next-generation sequencing (NGS) has already found its application in plant developmental biology. This review summarizes recent applications of NGS in understanding key questions in plant development, such as organ formation and genetic information transmission in germline cells. NGS has been used to study gene expression, transcription factor (TF) binding, and epigenetic regulation, which includes DNA methylation, histone modification, and profiles of small RNA (smRNA) expression. NGS has been combined with traditional and novel technologies to address these types of questions that have intrigued plant developmental biologists. This review will cover recent advances in cell-specific analysis of transcription by NGS, and their applications to understand organ formation and development in two model species Arabidopsis thaliana and maize (Zea mays) read more ...