An Overview of Next-generation Genome Sequencing Platforms
Chandra Shekhar Pareek
from: Next-generation Sequencing: Current Technologies and Applications (Edited by: Jianping Xu). Caister Academic Press, U.K. (2014)
The next-generation genome sequencing platforms are principally based on the immobilization of the DNA samples onto a solid support, cyclic sequencing reactions using automated fluidics devices, and detection of molecular events by imaging. This chapter reviews the platform-specific sequencing chemistries, instrument specifications, and general workflows and procedures of both the popular second generation sequencing platforms: GS FLX by 454 Life Technologies / Roche Applied Science, genome analyser by Solexa / Illumina, SOLiD by Applied Biosystems, and the third generation sequencing platforms: semiconductor sequencing by Ion Torrents / Life Technologies, PacBio RS by Pacific Biosciences, HeliScope™ single molecule sequencer by Helicos Bioscience Cooperation, Nano pore sequencing by Oxford Nanopore Technologies read more ...