Molecular Diagnostics: Current Research and Applications
Sophie Collot-Teixeira, Philip Minor and Robert Anderson
from: Molecular Diagnostics: Current Research and Applications (Edited by: Jim Huggett and Justin O'Grady). Caister Academic Press, U.K. (2014)
Molecular techniques, based on the detection of viral genomes, are revolutionising the field of diagnostic virus detection. They are currently replacing more traditional detection methods, such as cell culture, because they offer the multiple advantages of speed, sensitivity and specificity, which together, are not possible with other established techniques. Molecular methods afford the opportunity to detect viral genome(s), quantify viral load and detect different viral genotypes or mutations. Instruments and chemistries are becoming reasonably affordable and enclosed automated nucleic acid extraction/detection techniques are becoming more and more available increasing the throughput, the reproducibility and confidence in the results read more ...