Microbial Diagnostic Microarrays for Detection and Typing of Water-borne Pathogens
from: Microarrays: Current Technology, Innovations and Applications (Edited by: Zhili He). Caister Academic Press, U.K. (2014)
Even nowadays water quality assessment relies on the detection of so called indicator organisms (e.g. E. coli, coliforms, Enterococci, heterotrophic plate count) and utilizes cultivation-based methods. But it is waterborne pathogens that pose a major threat to human health, and the correlation between indicator organisms and pathogens was shown to be questionable. Therefore, having the possibility to detect pathogens directly would be of great advantage for water quality monitoring. However, detection of pathogens in an environment (i.e., water) requires a diagnostic tool of high specificity and sensitivity, with multiplexing potential and high degree of robustness. Microbial diagnostic microarrays (MDMs) present one possibility of addressing these requirements, and many promising microarray systems for the detection or typing of waterborne pathogens were developed and validated over past years. The current state of the art and needs for further developments will be discussed read more ...