Applications of Phylogenetic Microarrays to Profiling of Human Microbiomes
Oleg Paliy and Vijay Shankar
from: Microarrays: Current Technology, Innovations and Applications (Edited by: Zhili He). Caister Academic Press, U.K. (2014)
Human associated microbial communities are known to be highly diverse, comprising between hundreds to a thousand species, depending on the body area. The sheer numbers of species as well as the fastidious nature of most of these organisms make culture-based techniques both inefficient and challenging to study these communities. As a result, analyses of such communities are best accomplished by the use of high-throughput molecular methods such as phylogenetic microarrays and next generation sequencing. Phylogenetic microarrays have recently become a popular tool for the compositional analysis of complex microbial communities, owing to their ability to provide simultaneous quantitative data for many community members. This chapter will focus on currently available phylogenetic microarrays for the interrogation of human-associated microbiota, the technologies used to construct the arrays, as well as several key features that distinguish them from other approaches. We will also discuss optimization strategies for the development and usage of phylogenetic microarrays as well as opportunities to complement microarray analysis with other techniques read more ...