Bioleaching Microarrays for Profiling Microbial Communities in Acid Mine Drainage and Bioleaching Ecosystems
Huaqun Yin and Xueduan Liu
from: Microarrays: Current Technology, Innovations and Applications (Edited by: Zhili He). Caister Academic Press, U.K. (2014)
The dissolution of sulfide minerals yields hot, sulfuric acid-rich solutions generally referred to as ‘acid mine drainage' (AMD) that contains high concentrations of toxic metals. The resulting AMD may contaminate surrounding ecosystems. However, there populate a diverse range of microbes being utilized for "bioleaching" process, in which the use of acidophilic microorganisms to dissolve metals from mineral ores for subsequent recovery. Therefore, to deepen the understanding of ecosystem functioning and bioleaching mechanisms, it is necessary to study the diversity, composition, structure, function, and dynamics of microbial communities from AMD and bioleaching systems as well as the gene function, regulation and network of acidophilic microorganisms. In this chapter, we focus on the development and application of four types of bioleaching microarrays for analyzing microbial communities and isolated microorganisms from AMD and bioleaching systems. Also, we discuss the challenges and future directions of bioleaching microarray technologies read more ...