GeoChip Analysis of Soil Microbial Community Responses to Global Change
Kai Xue, Zhili He, Joy D. Van Nostrand, and Jizhong Zhou
from: Microarrays: Current Technology, Innovations and Applications (Edited by: Zhili He). Caister Academic Press, U.K. (2014)
Global climate change resulting from anthropogenic activities has become a great concern for human society, which would affect ecosystems deeply. Although responses of plant communities to global change factors have been well established, influences on belowground soil microbial communities still remains poorly understood, at least partly due to the challenges of analyzing soil microbial communities by conventional molecular tools. However, the development of metagenomic technologies, including microarray-based GeoChip, have overcome these limitations and revolutionized microbial research. GeoChip technology allows us to investigate the ecological functions of soil microbial communities involved in important biochemistry cycles. It has been utilized to investigate responses of soil microbial communities to several climate change factors, including elevated atmospheric CO2 concentrations, rising temperature, and increased O3 concentrations. Here, we summarize the application of GeoChip in these studies and discuss how information obtained from GeoChip enhances our understanding of ecological consequences of global changes. Moreover, the challenges of this technique will also be discussed read more ...