Microarray Data Analysis
Ye Deng and Zhili He
from: Microarrays: Current Technology, Innovations and Applications (Edited by: Zhili He). Caister Academic Press, U.K. (2014)
Microarray technology allows to examine the existence or expression levels of thousands to millions genes simultaneously in a small space but generates massive amounts of signal intensity data after the image scanning. How to extract and organize these signal intensity data to reflect true concentrations and how to dig into these complicate data for mining useful information are of great challenge. This chapter focuses on microarray data analysis, especially for environmental samples, and it includes a brief tutorial for the basic analysis steps, and basic statistical methods. This chapter is organized into three major sections: (1) microarray image processing for data extraction; (2) true spots determination, paired-comparison ratio and data normalization for data preprocessing; and (3) statistical analysis and network analysis for data mining read more ...