Microarrays for Microbial Community Analysis at a Glance
Zhili He and Jizhong Zhou
from: Microarrays: Current Technology, Innovations and Applications (Edited by: Zhili He). Caister Academic Press, U.K. (2014)
Microorganisms are the most diverse group of organisms, and play important and distinctive roles in their ecosystems, such as biogeochemical cycling of carbon, nitrogen, sulfur, phosphorus and metals, and biodegradation or stabilization of environmental contaminants. They also interact with their peers and/or other organisms (e.g., plants, animals) to form a complicated food web, significantly impacting ecosystem functions and services. However, understanding the diversity, composition, structure, functions, activities and dynamics of microbial communities remains challenging. Over the past decade, microarray-based technologies have been developed to address such a challenge. In this chapter, we provide an introduction of various microarrays for microbial community analysis, describe crucial issues in applications of microarray technologies for addressing fundamental scientific questions, and highlight their recent applications in environmental microbiology. In addition, we discuss the advantages and limitations of microarray technologies compared to high throughput sequencing technologies as well as challenges and future directions read more ...