Identification, Typing and Susceptibility Testing of Fungi (incl. Yeasts) by MALDI-TOF MS
Anna Kolecka, Maurizio Sanguinetti, Teun Boekhout and Brunella Posteraro
from: MALDI-TOF Mass Spectrometry in Microbiology (Edited by: Markus Kostrzewa and Sören Schubert). Caister Academic Press, U.K. (2016) Pages: 49-78.
Abstract
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has developed into a robust tool for the routine identification of many microbes, including bacteria, yeasts and filamentous fungi, which remain relevant for maintaining the quality of the human lifestyle, e.g. related to health, food, industry, ecology, water quality, waste management, and others. The number of correct and erroneous results depends mainly on the sample preparation method, the number of reference spectra present in the databases and the cut-off values used. These studies confirmed that MALDI-TOF MS platform outperformed traditional identification methods for fungi. Examples will be given on the use of MALDI-TOF MS for the identification of yeasts and filamentous fungi, including strain typing and susceptibility testing read more ...
