PCR Devices Using Glass Substrate
Hao Yu, Jianhua Qin and Bingcheng Lin
from: Lab-on-a-Chip Technology (Vol. 2): Biomolecular Separation and Analysis (Edited by: Keith E. Herold and Avraham Rasooly). Caister Academic Press, U.K. (2009)
Polymerase chain reaction (PCR) provides an in vitro method for rapid enzymatic amplification of fragments of DNA and it is one of the important analytical tools in biological sciences that benefit from miniaturization. In the past few years, the possibility of performing fast and small volume PCR on a single device has attracted great interest and witnessed great advances. The miniaturization of PCR has been demonstrated to have distinct advantages including small sample requirement, short amplification time, rapid heating/cooling rates, and the potential to integrate other functional components to reduce power consumption and improve portability. In this chapter, the motivation of underlying miniaturized format of PCR will be firstly introduced. Moreover, the recent research and progress on chip substrates, chip design, surface modification, and the characteristics of two types of microchip PCR are summarized. Several issues related to PCR reaction volume, heating methods, amplification speed, and the product-detection methods are discussed. Furthermore, the example applications of these methods to perform miniaturized format of PCR on a glass substrate are demonstrated. In addition, the potential applications of microchip-PCR to integrate functional components such as pre-PCR and post-PCR, and other practical issues related to implementation of the miniaturized devices are discussed read more ...